our species who we are how we are built all of this is embedded in our genetic code by understanding genetic variations across multiple individuals we gain valuable Insight that can improve our understanding of human health and disease with the Infinium assay from alumina you can measure these variations across a large number of individuals giving you a deeper understanding of how they impact different traits and diseases an individual Infinium assay genotypes a Locus using two color readouts one color for each Al the secret is in the combination of the Infinium assay with aluminous bead chip technology
on the surface of of each bead chip hundreds of thousands to millions of genotypes for a single individual can be assayed at once these tiny silica beads are housed in carefully etched microw Welles the beads are coated with multiple copies of an oligonucleotide probe that targets a specific Locus in the genome as DNA fragments pass over the bead chip each probe will bind to a complimentary sequence in the sample DNA stopping one base before the locus of Interest a specificity is conferred by a single base extension that incorporates one of four labeled nucleotides natural competition
among four bases minimizes bias allowing the pmer Rays to extend the probe with a correct base matching the target DNA once laser excited the nucleotide label emits a signal that's detected by an aluminous scanner intensity values for each color convey information about the alic ratio of a given Locus for example if the color representing a is approximately as intense as the color representing G the interpretation is that the genotype at that Locus is AG the data can be seamlessly analyzed using a luminous genome Studio software to call genotypes and evaluate copy number variation across the
genome when the assay data from a number of individuals are plotted distinct patterns emerge samples that have identical genotypes at an assade Locus exhibit similar signal profiles for diploid organisms viic Losi are expected to exhibit three clusters this proven chemistry produces unrivaled accuracy Superior call rates and the most consistent reproducibility [Music] this approach is already greatly advancing our understanding of genetics empowering you to find associations that will provide important insights across a range of research areas find out how the alumina Infinium asay can add a deeper understanding to your study
