Erewhon could back him cervezas here let's talk about chromatography the term CRO Metro means color and graphy means to write chromatography was first invented by a scientist named Mikhail Tess FET a design an experiment in which he packed a column with calcium carbonate he then added extract obtained from crush leaf along with a mixture of ether and ethanol he found that as a leaf extract passed down the column the chlorophyll pigments present in the leaf extract got separated the calcium carbonate pack in the column is known as stationary phase the mixture of ether and ethanol
is known as the mobile phase the mobile phase moves down the column along with the sample the leaf extract or the sample used is term as the analyte the colorful separation of chlorophyll pigments so obtained is known as the chromatogram now besides all these terms there are also some similar terms used in chromatography so let's see each of them mobile phase is also known as eluent the mobile phase leaving the column is known as illy weight in a sample that is coming out from the column is known as in divide let's see how chromatography works
when the leaf extract is applied on the column the chlorophyll pigments present in the sample are adsorbed on the stationary phase next when the mobile phase is passed down the column the chlorophyll molecules have two options either to remain in the stationary phase or to enter the mobile phase the molecules that are more soluble in mobile phase enters the mobile phase and most fast down the column while the molecules that are less soluble in mobile phase spends more time on the stationary phase and move slowly hence separation occurs due to the differential partitioning of sample
between the mobile phase and the stationary phase [Music] there are different types of chromatography such as paper chromatography thin layer chromatography column chromatography affinity chromatography gel permeation chromatography gas chromatography etc these all will be discussed in the upcoming videos [Music]
